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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
26/09/2014 |
Actualizado : |
18/03/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
IBÁÑEZ, F.; REZANO, A.; FREDES, A.; RODRIGUEZ, G.; REGGIO, A.; VILARÓ, F. |
Afiliación : |
FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANABELA REZANO MATKOVICH, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALEJANDRO DAVID FREDES SIVOPLAS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO ADOLFO RODRIGUEZ TEXEIRA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA MARIA REGGIO BIGANZOLI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Development of a rapid microplate assay for determining pungency in onion breeding program. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Acta Horticulturae (ISHS), 2012, no. 935, p. 61-65. |
ISSN : |
0567-7572 |
DOI : |
10.17660/ActaHortic.2012.935.8 |
Idioma : |
Inglés |
Notas : |
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. |
Contenido : |
ABSTRACT.
Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). |
Thesagro : |
AJO. |
Asunto categoría : |
-- |
Marc : |
LEADER 02138naa a2200229 a 4500 001 1050611 005 2022-03-18 008 2012 bl uuuu u00u1 u #d 022 $a0567-7572 024 7 $a10.17660/ActaHortic.2012.935.8$2DOI 100 1 $aIBÁÑEZ, F. 245 $aDevelopment of a rapid microplate assay for determining pungency in onion breeding program.$h[electronic resource] 260 $c2012 500 $aXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. 520 $aABSTRACT. Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). 650 $aAJO 700 1 $aREZANO, A. 700 1 $aFREDES, A. 700 1 $aRODRIGUEZ, G. 700 1 $aREGGIO, A. 700 1 $aVILARÓ, F. 773 $tActa Horticulturae (ISHS), 2012, no. 935, p. 61-65.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
21/02/2014 |
Actualizado : |
22/07/2021 |
Tipo de producción científica : |
Documentos |
Autor : |
ZERBINO, M.S.; CHIARAVALLE, W. |
Afiliación : |
MARIA STELLA ZERBINO, CIAAB (Centro de Investigaciones Agrícolas "Alberto Boerger"). |
Título : |
Importancia del daño causado por el "Barrenador de los brotes" : epinotia aporema (Wals.) en distintos estados fenologicos. |
Fecha de publicación : |
1985 |
Fuente / Imprenta : |
ln: Jornada de Cultivos de Verano, 1985 set 4 : La Estanzuela [Memorias]. Montevideo (Uruguay): CIAAB, 1985. |
Páginas : |
p.5-12. |
Idioma : |
Español |
Thesagro : |
DAÑOS; EPINOTIA APOREMA; FACTORES DE RENDIMIENTO. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15876/1/CIAAB.-Jornada-Cultivos-de-Verano.1985.pdf;verano#page=7
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Marc : |
LEADER 00590naa a2200169 a 4500 001 1045765 005 2021-07-22 008 1985 bl uuuu u00u1 u #d 100 1 $aZERBINO, M.S. 245 $aImportancia del daño causado por el "Barrenador de los brotes"$bepinotia aporema (Wals.) en distintos estados fenologicos. 260 $c1985 300 $ap.5-12. 650 $aDAÑOS 650 $aEPINOTIA APOREMA 650 $aFACTORES DE RENDIMIENTO 700 1 $aCHIARAVALLE, W. 773 $tln: Jornada de Cultivos de Verano, 1985 set 4 : La Estanzuela [Memorias]. Montevideo (Uruguay): CIAAB, 1985.
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